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In particular, twophoton excitation excels at imaging of living cells, especially within intact tissues such as brain slices, embryos, whole organs, and even entire The application of twophoton excitation to fluorescence microscopy has become a powerful tool for studying biological function in live tissue and offers many Experiments deeper in tissue benefit from twophoton excitation 2PE; also referred to as twophoton, or multiphoton microscopy, which allows highresolution and highcontrast fluorescence microscopy deep in the brain Denk et al.

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